CenBRAIN's New Publication in MDPI Biosensors

Titled“On-Site Biolayer Interferometry-Based Biosensing of Carbamazepine in Whole Blood of Epileptic Patients”, this contribution has been published in theBiosensorsjournal. In this publication, we present two types (being indirect vs. direct) of fiber-optic biolayer interferometry (FO-BLI) biosensors for on-site carbamazepine monitoring. The work proves that Direct FO-BLI is more effective for integration into the clinic by delivering carbamazepine values from whole blood within minutes. Congratulations toSumin Bian, Research Assistant Professor, and to this paper’s co-authors for this excellent achievement.

Citation

Bian, S.; Tao, Y.; Zhu, Z.; Zhu, P.;Wang, Q.;Wu, H.; Sawan, M. On-Site Biolayer Interferometry- Based Biosensing of Carbamazepine in Whole Blood of Epileptic Patients. Biosensors 2021, 11, 516.

Abstract

On-site monitoring of carbamazepine (CBZ) that allows rapid, sensitive, automatic, and high-throughput detection directly from whole blood is of urgent demand in current clinical practice for precision medicine. Herein, we developed two types (being indirect vs. direct) of fiber-optic biolayer interferometry (FO-BLI) biosensors for on-site CBZ monitoring. The indirect FO-BLI biosensor preincubated samples with monoclonal antibodies towards CBZ (MA-CBZ), and the mixture competes with immobilized CBZ to bind towards MA-CBZ. The direct FO-BLI biosensor used sample CBZ and CBZ-horseradish peroxidase (CBZ-HRP) conjugate to directly compete for binding with immobilized MA-CBZ, followed by a metal precipitate 3,30-diaminobenzidine to amplify the signals. Indirect FO-BLI detected CBZ within its therapeutic range and was regenerated up to 12 times with negligible baseline drift, but reported results in 25 min. However, Direct FO-BLI achieved CBZ detection in approximately 7.5 min, down to as low as 10 ng/mL, with good accuracy, specificity and negligible matric interference using a high-salt buffer. Validation of Direct FO-BLI using six paired sera and whole blood from epileptic patients showed excellent agreement with ultra-performance liquid chromatography. Being automated and able to achieve high throughput, Direct FO-BLI proved itself to be more effective for integration into the clinic by delivering CBZ values from whole blood within minutes.

More information can be found at the following link:

https://doi.org/10.3390/bios11120516

Fig.1: he principle of on-site CBZ monitoring for the (A) indirect FO-BLI biosensor via indirect competitive binding and (B) direct FO-BLI biosensor via direct competitive binding.